Efficacy

Primary Pharmacodynamics and efficacy studies (in vivo, ex vivo and/or in vitro) are intended to investigate the mode of action and/or efficacy of a substance in relation to its desired therapeutic target. Such studies are generally conducted during the discovery phase and can contribute to dose selection for both nonclinical and clinical studies.

Therapeutic Areas

In vitro assays

In vivo assays

Alzheimer’s disease

Amnesia

  • Scopolamine-induced amnesia model

Memory test evaluation

Parkinson disease

  • Rotenone-induced model
  • Reserpine-induced model
  • 6-OHDA-induced model

Multiple sclerosis

  • MOG- induced Experimental Autoimmune Encephalomyelitis (EAE)

Epilepsy

  • Pilocarpine induced seizures
  • Pentylenetetrazole induced seizures

Anxiety and Depression

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

Ex vivo assays

In vivo assays

  • Ovalbumin (OVA)-induced Allergic Asthma Model
  • Carrageenan-induced pleurisy in mice

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

In vivo assays

Intestinal inflammation

Ulcers (acute and chronic)

Acid gastric secretion evaluation

Intestinal transit

Gastric emptying

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

In vivo assays

Psoriasis

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

In vivo assays

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

In vivo assays

General inflammation models

  • Ear edema (arachidonic acid– and croton oil-induced edema)
  • Paw edema (carrageenan-mice, carrageenan -rats, complete Freund’s adjuvant (CFA) – and zymosan- induced edema)
  • Peritonitis (carrageenan-, zymosan- and lipopolysaccharide (LPS)- induced peritonitis)
  • Pleurisy (carrageenan-, zymosan- and lipopolysaccharide (LPS)- induced pleurisy)

Arthritis and osteoarthritis

  • CFA-induced arthritis
  • Methylated bovine serum albumin (mBSA)-induced arthritis
  • Monosodium iodoacetate-induced osteoarthritis

General nociception models

  • Nociception induced by chemical agents
  • Surgical incision-induced nociception (post -operative pain)
  • Hyperalgesia induced by chemical agents (carrageenan, PGE2, bradykinin, 5-HT, pro-inflammatory cytokines)
  • Hyperalgesia induced by thermal stimulus

Neuropatin pain

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

Ex vivo assays

  • Isolated heart
  • Isolated veins and arteries
  • Isolated bladder
  • Muscle contraction and relaxation
  • Intraventricular pressure

In vivo assays

Blood pressure and heart rate

Hypertension

  • Hypertension (two-kidney, one-clip – 2K1C)

Cistitis

  • Cyclophosphamide-induced hemorrhagic cystitis

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.

In vitro assays

In vivo assays

Diabetes

Obesity

  • High fat diet-induced obesity

Body growth

Pharmacodynamic

The pharmacodynamic studies are important for evaluating the mechanism of action of a new compound. The development of each compound/product may require a different approach; therefore, CIEnP offers personalized service for experimental trials. We have various techniques (Agonists and antagonists, colorimetric assay, flow cytometry, ELISA, histology, immunohistochemistry, immunofluorescence, real-time PCR, western blot and others), equipment, methods, and approaches available to be associate to different experimental models.