Cell-Based Assays
CIEnP provides in vitro screening and mechanism of action studies using primary or immortalized cell lines. Our scientific team provides a broad array of validated assays as well as in-depth biology support for drug discovery and we work with sponsors to determinate the best in vitro assay for the proposal.
Cell Lines
Lung carcinoma – A549
Colorectal adenocarcinoma – Caco-2
Human Embryonic Kidney – HEK-293
Keratinocytes – HEKn
Fibroblasts – HDFn
Melanocytes – HEMn-MP
* Others on request
Ex vivo cell subsets
Bronchoalveolar lavage cells;
Macrophages; Splenocytes;
Lymphocytes;
Primary neuronal cultures from Sprague Dawley rats;
Primary glial cultures from Sprague Dawley rats;
* Others on request
Assays offered
- Cytotoxicity
- Apoptosis
- Cell cycle
- Clonogenic assay
- Biomarkers (quantitative PCR or ELISA)
- Cell proliferation
- Cell stimulation/activation
- Cytokine levels
- Cellular invasion/migration
- Cellular phenotyping (multi-color flow cytometry)
- Mitochondrial membrane potential
- Oxidative stress
- Morphological characterization
- Enzymatic measurements
* Others on request
Skin
Human skin
Synthetic membrane
Assay offered
- The in vitro release test (IVRT) using a Vertical Diffusion System (Franz Cell)
- In vitro skin permeation using a Vertical Diffusion System (Franz cell)
The above list is a small sampling of the available assays. Contact a scientist today to discuss a cell-based assay test that can meet your primary objectives.